PCF11 and neuroblastoma: To this end, we generated stable cell lines expressing an Isopropyl β-d-1-thiogalactopyranoside (IPTG)-inducible shRNA against PCF11 (further details see Methods section). Interestingly, PCF11 depletion abrogates WNT signalling in reporter assays (Fig. 4c), and results in cell cycle retardation, increased rate of apoptosis, reduced cell proliferation (Fig. 4d–f, Supplementary Figure 6b) and ultimately differentiation of neuroblastoma (Fig. 4g).