The greater in vitro antitumor effect in the presence of anti‐adenovirus antibodies than without these antibodies might be a result of the more efficient non‐adenovirus receptor‐mediated infection by adenovirus‐containing cell fragments formed after cell lysis by oncolytic adenovirus.14 In the present study, EHMK‐51‐35 carrier cells infected with AdE3‐midkine and Ad‐cGM‐CSF showed 100% complete tumor reduction of subcutaneous mouse ovarian tumors and 60% complete tumor reduction of intraperitoneally metastasized mouse ovarian tumors. The gene discussed is MDK; the disease is neoplasm.