CSF2 and infection: These side effects are considered to be a result of DIC by cell lysis of carrier cells; it appears that the nuclear membrane was already broken at the time of freeze–thaw and the carrier cells were not alive before administration.15 By reducing the infectious dose and infection time of AdE3‐midkine and Ad‐GM‐CSF or by using fresh carrier cells without freezing, it may be possible to administer living carrier cells, thereby reducing side effects and increasing the dose.