However, EHMK‐51‐35 carrier cells infected with AdE3‐midkine or co‐infected with AdE3‐midkine and Ad‐mGM‐CSF induced 20% or 60% of complete tumor reduction, respectively, which increased the survival rates significantly compared to controls and A549 carrier cells (p < 0.05). This evidence concerns the gene MDK and neoplasm.