Consequently, we proposed that GSK3β/β-catenin is likely involved in regulating glucose metabolism and proliferation in cervical cancer with mutant PIK3CA. In the present study, the high expression and nuclear translocation of β-catenin was observed in cervical cancer cells and tumor tissues with mutant PIK3CA. The downregulation of β-catenin rapidly decreased the proliferation, glucose uptake, and lactate production of tumors with mutant PIK3CA. The expression of GLUT4 and LDHB were abated following the reduction of β-catenin. This evidence concerns the gene PIK3CA and neoplasm.