Furthermore, we analyzed the expression of β-catenin, SIRT3, GLUT4, and LDHB by immunohistochemistry in xenografts with wild-type and mutant PIK3CA. The expression levels of β-catenin, GLUT4, and LDHB in xenograft models with PIK3CA E545K were enhanced, while that of SIRT3 was decreased compared with those in the corresponding controls, which was consistent with that in cervical cancer cells in vitro (Fig. 5e). The gene discussed is SIRT3; the disease is cervical cancer.