In conclusion, we demonstrate that PIK3CA E542K and E545K mutations induce glycolysis in cervical cancer cells through the induction of the β-catenin/SIRT3 signaling pathway (Fig. 7), which offers important implications for the underlying mechanism of PIK3CA E542K and E545K mutation-mediated glycolysis and proliferation, but also provide the new insights into the development of therapeutic approaches using PIK3CA E542K and E545K mutations as a target to prevent metastasis in various cancers, including cervical cancer. This evidence concerns the gene PIK3CA and cancer.