To evaluate the effect of PIK3CA E542K and E545K mutations on the alteration of glucose metabolism, we analyzed the level of glucose uptake and lactate production and found that cells with mutant PIK3CA had a higher turnover of glucose uptake and lactate production, indicating that PIK3CA E542K and E545K mutations enhanced the level of glycolysis in cervical cancer (Fig. 2c, d). This evidence concerns the gene PIK3CA and cervical cancer.