Because B10 and CD19+CD24hiCD27hi cells were significantly decreased in patients with AP, we investigated the expression of the activation markers CD80 and CD86 by immunofluorescence staining and flow cytometry to determine whether a difference was present in the activation status of B10 or CD19+CD24hiCD27hi cells between patients with AP and healthy individuals. Here, CD19 is linked to alkaline phosphatase measurement.