Both bFGF and CHIR99021 could individually promote the proliferation of the cells compared with BM from day 5 onwards (P < 0.001), and the combination of bFGF and CHIR99021 (BC) significantly improved cell proliferation from day 5 onwards compared with the treatment of CHIR99021 (P < 0.001) (Fig. 2a). The gene discussed is FGF2; the disease is breast cancer.