Exogenous Irf6 significantly increased apoptosis of MCF-ErbB2 cells in 3D culture (Fig. 3d) and noticeably reduced their clonogenicity without adhesion to the ECM in soft agar (the ability to grow in agar is a well-known consequence of cancer cell anoikis resistance [11]) (Fig. 3e). Here, IRF6 is linked to cancer.