Based on previous reports that ARRDC3 is a negative regulator of ITG β4 signaling [23] and its expression is epigenetically silenced in TNBC cells [25,26], we assessed the effects of modulation of ARRDC3 expression on ubiquitination of ITG β4 and its intracellular trafficking pattern in multiple subtypes of breast cancer cells. Here, ARRDC3 is linked to breast carcinoma.