In summary, the decreased expression of c-Myc, HIF-1α and the phosphorylated NF-κB- RelA/p65 subunit and P-AMPK, affecting related pathways as does the mTOR signalling pathway, and the increased expression of p53 in si-hVDAC1-TTs agree with their regulation of cancer cell metabolism. This evidence concerns the gene TP53 and cancer.