Based on these results, melanoma cells were exposed for 24–48 h to 250–500 μM Ole and the level of apoptosis, assessed uisng western blotting of PARP1, confirmed that only 500 μM Ole was able to promote the expression of a significant level of cleaved PARP1 after 48 h of treatment (Figure S2c). Here, PARP1 is linked to melanoma.