APAF1 and central nervous system cancer: To better understand the mechanism of miR-144-3p/TOP2A affected cell growth and invasive ability of HCMV-infected glioma cells, the apoptosis and metastasis-related proteins (Caspase-3, Bcl-2, Bax, Apaf-1, MMP, TIMP, Geletinase, Calpain, Cathepsin and etc.)should be detected in the further study.