Further functional assays indicated that ZFAS1 overexpression inhibited cell migration and invasion by regulating epithelial.[29] Other studies reported that overexpression of ZNFX1-AS1 inhibited the cell proliferation and colony formation in HCC cell lines and also induced HCC cell apoptosis via regulating the methylation of miR-9.[8] In breast cancer, knockdown of cytoplasmic ZFAS1 by siRNA in mouse mammary epithelial cell lines increases proliferation.[30] No studies have reported that ZFAS1 exerts tumor suppressor function in other tumors by regulating protein expression. The gene discussed is ZNFX1; the disease is hepatocellular carcinoma.