As shown in Figure 3D there was no difference in Iba1+cells (measured as % of Iba+ staining per tumor area) in wt and cxcr6ko mice (cxcr6ko: 0.69 ± 0.02%; wt: 0.72 ± 0.04%; n = 4, p = 0.56; Student's t-test), but there was a strong reduction in CD68+cells (measured as % of CD68+ staining per tumor area) in cxcr6ko mice compared to wt mice (53% reduction, cxcr6ko: 0.26 ± 0.02%; wt: 0.55 ± 0.02%; n = 4, p < 0.05; Student's t-test), indicating that although there was no difference in the recruitment of total GAMs in tumor mass, they were differently activated in cxcr6ko mice. Here, AIF1 is linked to neoplasm.