Specifically, we demonstrated that: (i) GL261 cells express both CXCL16 and CXCR6; (ii) stimulation with CXCL16 promotes GL261cell migration, invasion, and proliferation; (iii) the silencing of CXCR6 on glioma cells reduces their proliferation rate and migration ability; (iv) in vivo, transplantation of CXCR6-silenced GL261 cells in wt mice leads to a reduced tumor cell proliferation and infiltration and tumor volume compared to mice injected with not silenced glioma cells. Here, CXCL16 is linked to neoplasm.