To study the role of CXCL16/CXCR6 in glioma development in a mouse model, we analyzed cxcl16 and cxcr6 mRNAs expression in GL261, and in the more aggressive derived glioma stem cells (GL261/cd133+ cells): as shown in Figure 2B, both chemokine and its receptor are expressed by GL261 cells, with higher expression of cxcr6 in GL261/cd133+ cells (both by RT- PCR and RT-qPCR analysis). This evidence concerns the gene CXCL16 and glioma.