ACTA1 and Hepatic fibrosis: Comparison analysis between the TGF-β effect at 2 vs 48 hours in HSCs showed greater activation of the hepatic fibrosis/HSC activation pathway (Figure 2D), which correlated with up-regulation of key myofibroblastic and fibrogenic genes such as α-smooth muscle actin (α-SMA) (logFC, 2.785; P = 1.25E-161), fibronectin 1 (logFC, 2.860; P = 5.95E-111), or collagen 1α1 (logFC, 1.002; P = 2,04E-19) at 48 hours (Figure 2E).