SCAF11 and colitis: To determine whether the increased intestinal inflammation in Casp11−/− mice led to increased colitis-associated colorectal tumourigenesis, Casp11−/− mice and their wild-type (WT) littermates were treated with the DNA methylating agent AOM (12.5 mg/kg) and three cycles of 2% DSS interspaced with normal drinking water (Fig. 1a).