In support of these experimental findings, in silico Pearson correlation analysis of human GBM specimens by R2 indicated that NDRG1 expression was anti-correlated to that of ASCL1 in the majority of GBM samples as well as in other different types of cancer (TCGA and PANCANCER datasets; Supplementary Fig. 4a–c). Here, NDRG1 is linked to glioblastoma.