While FOXM1 and TYMS expression was coordinately modulated in response to 5-FU treatment in the 5-FU sensitive CCA cell lines, TYMS expression remained comparatively high throughout the time course in the resistant HuCCA cells, indicating a potential uncoupling between FOXM1 and TYMS expression in the resistant HuCCA cells. This evidence concerns the gene TYMS and cholangiocarcinoma.