In contrast, upon infection of D. discoideum producing 2×PHFAPP-mCherry and Arf1-GFP with L. pneumophila ΔicmT, the Golgi membranes were inevitably brought into proximity of the compartment harboring the bacteria but did not engage in sustained interactions (Fig. 5B; see also Movie S9). The gene discussed is ARF1; the disease is infection.