In addition, circADAMTS13 overexpression increased the percentage of apoptotic cells (Annexin V+/PI+ and Annexin V+/PI− cells) compared with mock cells (P < 0.05) according to the flow cytometric analysis, suggesting the regulatory effects of circADAMTS13 on cell apoptosis (Fig. S2A,B); however, the migration and invasion ability of HCC cells was not significantly changed (data not shown). This evidence concerns the gene ANXA5 and hepatocellular carcinoma.