Therefore, we measured this protein in all cases (<i>n</i> = 30) and controls selected (<i>n</i> = 90).<h4>Results</h4> There were no differences in the plasma TIMP-4 levels of cases compared with controls (1,144 ± 263 versus 1,160 ± 362 pg/mL, respectively; <i>p </i>> 0.05).<h4>Conclusion</h4> Plasma TIMP-4 levels were not altered at 20 to 25 weeks of gestation, before the manifestation of clinical symptoms; therefore, they are not good predictors of the development of preeclampsia. This evidence concerns the gene TIMP4 and preeclampsia.