Based on previous observations of methylation changes in canine DLBCL [29], the present study aimed to corroborate, using in vitro and in vivo approaches, the promoter methylation of four TSGs (HOXD10, FGFR2, ITIH5 and RASAL3), whose mRNA expression was confirmed to be significantly reduced in DLBCL samples vs controls (S1 Fig). The gene discussed is RASAL3; the disease is diffuse large B-cell lymphoma.