In another approach using an in vivo model, we injected glioblastoma cells into the skin of nude mice, and after 2 days, the mice received a single intravenous injection of adenovirus encoding human SEMA3F (Ad-3F) or a control adenovirus (Ad-Cont) (Nakayama et al., 2015a). The gene discussed is SEMA3F; the disease is glioblastoma.