We performed analysis of cancer cell stemness markers (ALDH1A1, CEACAM5, ALCAM, LGR5, DPP4, CD133, CD24, CD29, and CD44), epithelial-mesenchymal transition (EMT) markers (SNAIL1, SNAIL2, CDH1, and CDH2) and multipotency markers (NANOG and POU5F1) in HCT116 and DLD-1 cell 2D and 3D cultures in the absence of treatment. This evidence concerns the gene CD44 and cancer.