To test the feasibility of this approach in the murine model of Hurler syndrome, male and female mice were injected via the tail vein with 1.5 × 1011 vector genomes (vg) of each AAV2/8 ZFN targeting intron 1 of the albumin locus and 1.2 × 1012 vg of an AAV2/8 hIDUA promoterless donor construct and evaluated over 4 months. The gene discussed is ALB; the disease is Hurler syndrome.