TNF and neoplasm: Moreover, tumor tissue-derived TIM-1+B cells were potent suppressors of CD8+ T cell effector functions, as the TNF-α and IFN-γ production of CD8+ effector T cells was significantly decreased after coculture with TIM-1+ B cells or without depleting TIM-1+B cells (Fig. 2c, and d and Additional file 8: Figure S2).