Additionally, we identified that the TIM-1 ligand TIM-4 was mainly expressed on myeloid cells, which were determined to come into contact with B cells in the HCC microenvironment, suggesting that myeloid cells could induce TIM-1+ Breg cells to produce IL-10 through TIM-1/TIM-4 signaling. Here, IL10 is linked to hepatocellular carcinoma.