In our study, EGFR genotyping using EV-derived DNA and cfDNA from the supernatant of pleural effusions in patients who were either EGFR-TKI naïve or who acquired resistance to EGFR-TKI showed good agreement with tissue EGFR genotyping (κ =0.861, 0.810, 1.0, 0.885). Here, EGFR is linked to Pleural effusion.