EGFR and Pleural effusion: EGFR genotyping using EV-derived DNA from the supernatant of the pleural effusions showed perfect agreement (19/19) with tissue EGFR genotyping across the 19 EGFR mutant cases, and this method identified three additional EGFR mutations (cases 20, 21 and 22) in cases in which tissue EGFR genotyping showed wild-type results.