First, in patients who were EGFR-TKI naïve, EGFR genotyping using EV-derived DNA from the supernatant of pleural effusions is in 100% agreement with tissue EGFR genotyping and has higher sensitivity, as this method was capable of detecting three additional mutant cases in patients who were otherwise diagnosed as tissue wild-type. This evidence concerns the gene EGFR and Pleural effusion.