Dessus-Babus et al. (2000) and Leonard et al. (2017) reported increased IL-6 secretion after chlamydial infection under CHX-influence, which we could confirm after comparing CHX-free and CHX-incubated conditions by t-test (data not shown). IL-8 secretion, however, was previously shown to decrease after CHX-treatment of Chlamydia-infected host cells (Dessus-Babus et al., 2000), which was not confirmed in our study. This evidence concerns the gene CXCL8 and chlamydia trachomatis infectious disease.