To estimate the transcript levels of gonadotropin-related genes, we performed real-time RT-PCR using specific primers for Fshr, Fshβ, and Lhr. Although the Fshr and Fshβ mRNA levels were significantly increased in rats with PCOS, these gonadotropin-related gene levels were significantly decreased in PCOS + EC500-treated rats compared to the corresponding rats with PCOS (Figures 5A,B). Here, FSHB is linked to polycystic ovary syndrome.