Univariate and multivariate Cox’s regression analysis (adjusted to tumor grade and tumor stage) showed a 2.2-fold and a 2.5-fold increased risk for tumor-associated death in the Piwi-like 2-positive group (P = 0.016 and P = 0.008; Table 3), Piwi-like 2 staining was again an independent factor for DSS in multivariate analysis. This evidence concerns the gene PIWIL2 and neoplasm.