To decrease the expression of TGF-β1 and TGF-β2, we infected human melanoma (A375) or pancreatic cancer (HPAC) cells with recombinant adenoviruses containing shRNA for TGF-β1 or TGF-β2 (shTGF-β1 or shTGF-β2, respectively) at various MOIs (10, 50, and 100). The gene discussed is TGFB1; the disease is melanoma.