IFNA1 and infection: Infection with Vpu-deficient HIV-1 (HIV-1LAIΔvpu) in IFN-treated Tetherin-KO cells shows increased virus release as compared to control cells (Tetherin_1 = 20.5 fold, Tetherin_2 = 14 fold in Figure 4F – left panel), confirming the late inhibition of Vpu-deficient HIV by Tetherin.