While establishing this mouse peritonitis model, we observed that detachment of primed iBMDMs was sufficient to induce activation of the inflammasomes in cells expressing full-length NLRP3 and in cells with miniNLRP3, which is not surprising as activation of NLRP3 inflammasome has been previously linked to changes in cell volume54 and activation of ion channels (reviewed in55). Here, NLRP3 is linked to peritonitis.