As shown in Supplementary Tables S2, more breast cancer patients were stage III or IV (39 out of 86, 45.3%) when Twist1 displayed a high expression level, while fewer breast cancer patients were at these stages (7 out of 45, 15.6%) when Twist1 displayed a low expression level (χ2 = 10.239, P = 0.001). The gene discussed is TWIST1; the disease is breast cancer.