Therefore, with the goal of better understanding the importance of SEMA6A in lung cancer cells, in vitro cell proliferation, clonogenic and apoptosis assays, and in vivo xenograft animal experiments were performed to examine the functions of SEMA6A. Moreover, different truncations of SEMA6A were overexpressed in lung cancer cells to study the functions of the parts of SEMA6A. Finally, SEMA6A-related signaling was delineated by co-immunoprecipitation, co-culture, and gene silencing assays. Here, SEMA6A is linked to lung cancer.