To explore the influence of changes in C5orf66-AS1 expression on the proliferation of cervical cancer cells, C5orf66-AS1 was down- or upregulated in cervical cancer SiHa and C-4 I cell lines via transfection with siRNA or the pcDNA3.1-C5orf66-AS1 over-expression plasmid. Here, PITX1-AS1 is linked to cervical carcinoma.