ANXA1 and neoplasm: To characterize the contribution of ANXA1-containing EVs on tumour aggressiveness, we compared the effects of the addiction of whole serum starved supernatants (SS), EV-depleted (EDS), and EV-enriched (EVs) fractions from both WT and ANXA1 KO MIA PaCa-2 cells on their own motility potential (Figure S1) and by adding the fractions from WT source cell line to ANXA1 KO recipient cells and viceversa (Figure 2A–D).