To determine whether c‐Fos O‐GlcNAcylation is altered under conditions simulating AD, we pulled down O‐GlcNAcylated proteins using wheat‐germ‐agglutinin (WGA)‐conjugated agarose beads (that well‐known to bind to O‐GlcNAc) in the brains of 5xFAD mice, a model of AD, and Aβ‐treated primary cortical neurons and SH‐SY5Y cells. Here, FOS is linked to Alzheimer disease.