The microarray comprised fragments of 91 antigens, including cancer-testis antigens, such as CTAG1B, MAGEA1, MAGEA3, DDX53, CTAGE5, SPAG8, SPAG6, and CSAG3, other known tumor antigens, such as TP53, MUC1, SOX2, BRCA2, TERT and BIRC5, and artificial peptides previously shown to elicit cancer-associated autoantibody responses (25–27). This evidence concerns the gene MAGEA1 and neoplasm.