There was a significant relationship between altered expression levels of NKX6.3, p53, AICDA, and EP300 proteins and clinicopathologic parameters, including depth of invasion (Chi-Square test: P = 0.0026, P = 0.2302, P = 0.039, and P = 0.0553, respectively), lymph node metastasis (Chi-Square test: P < 0.0001, P = 0.148, P = 0.4184, and P = 0.0889, respectively), and TNM stage (Chi-Square test: P < 0.0001, P = 0.1373, P = 0.0539, and P = 0.029, respectively). This evidence concerns the gene NKX6-3 and metastatic malignant neoplasm in the lymph nodes.