Liver metastasis was significantly increased in mice implanted with CCLP1-TSPAN1 cells compared to that of the control group, and the number of tumor nodules was dramatically decreased in mice implanted with HCCC9810-shTSPAN1 cells compared to that of shCon cells 4 weeks after operation (Fig. 3c). The gene discussed is PPFIBP2; the disease is neoplasm.