By using ESCC mouse xenograft model, we found miR-10b-3p promotes multiple aspects of tumor development, including tumor growth and metastasis, and we verified the inversed correlation between miR-10b-3p and FOXO3 by IHC assays with the ESCC mouse xenograft model and human ESCC tissue samples. Here, FOXO3 is linked to esophageal squamous cell carcinoma.