To investigate the role of SOX30 on cancer cell metastasis, we generated gain-of-function cell models in ADC cell lines (A549 and SPC-A-1) and SCC cell lines (H520 and H2170) by transfecting SOX30 or empty vector plasmids (Fig. 2a), and determined cell migration and invasion by transwell assays. Here, SOX30 is linked to cancer.