The effects of dacarbazine, an alkylating agent used as chemotherapy in human metastatic melanoma, vemurafenib, a BRAF inhibitor, and LY294002, a PI3K inhibitor, were evaluated for both canine oral melanoma cell lines (Ocr_OCMM1X & Ocr_OCMM2X) and two human cutaneous melanoma cell lines as controls (A375 and Sk-Mel-28, both BRAF V600E-mutated). The gene discussed is BRAF; the disease is metastatic melanoma.