In the cohort of one thousand five hundred and forty AML analyzed by Papaemmanuil and colleagues [2], among those mutated for NPM1, the most frequently-associated mutated genes were CHIP mutations—DNMT3A which was found mutated 54% of the time and TET2 in 16% of cases—, and mutations in tyrosine-kinase and RAS pathways genes, which were found in 39% of cases for FLT3, in 19% for NRAS, and 15% for PTPN11. Through variant allele frequency (VAF) analysis, the order of mutations showed that CHIP mutations appeared first, followed by NPM1, and then by mutations in signaling pathway genes. This evidence concerns the gene FLT3 and acute myeloid leukemia.