Therefore, in the present study we investigated the effects of ICV injected nesfatin-130-59, proposed to be the active core of nesfatin-1, on anxiety behavior using the open field test, elevated zero maze, and light/dark box as well as on anhedonic/depression-like behavior using the sucrose preference and the novelty-induced hypophagia test in normal weight rats. The gene discussed is NUCB2; the disease is major depressive disorder.