Interestingly, in contrast to the observed effects in normal weight rats, nesfatin-130-59 injected ICV did not induce behavioral alterations in DIO rats in neither of the anxiety—(elevated zero maze, open field, light/dark box) or anhedonia/depression-like—(novelty-induced hypophagia, sucrose preference test) assessing tools. Here, NUCB2 is linked to depressive symptom measurement.