Cell distribution in the cell cycle, the expression of CCND1, the activity of signaling pathways crucial for melanoma, including the RAF/MEK/ERK pathway, WNT/β-catenin pathway, and NF-κB signaling were very similar when different growth factors, HGF, EGF and bFGF, were used in the culture medium alone, in combination or were omitted. This evidence concerns the gene NFKB1 and melanoma.