ANO1 and head and neck squamous cell carcinoma: Furthermore, the results consistently showed that these three genes were frequently co-amplified (28%) and overexpressed at a higher frequency (39–46%), and, more importantly, these molecular alterations (in particular CTTN and ANO1 overexpression) were associated with a worse clinical outcome in the TCGA cohort of 279 HNSCC patients and also in an extended TCGA cohort which added 251 new HNSCC patients (n = 530) (Figure 5).