To characterize the enzymes responsible for the 2-AG hydrolytic activity in PBMCs from non-HD and HD individuals, lysates from untreated (i.e., naïve) cells were pre-incubated with either JZL184—an inhibitor of both MAGL and CES1 [29]—or WWL113—a selective inhibitor of CES1 [30] —prior to adding exogenous 2-AG substrate (Figure 2C). Here, CES1 is linked to Huntington disease.