FAS and steatosis: Our RT-PCR analysis revealed that the gene expression of PPAR-γ, SREBP-1c, FAS, SCD1, and FAT-P was upregulated in the liver of HFD mice compared to STD mice, confirming that HFD induced impairment of the lipid metabolizing gene expression that is involved in steatosis [4,6,57,58,59].