SIRT1 and gestational diabetes: It is noteworthy that the GDM/SIRT1(↑) and GDM/SIRT1(↔) groups differed with regard to the directionality of leukocyte ACLY expression; as it was previously mentioned, the leukocyte ACLY mRNA level was significantly down-regulated in the GDM/SIRT1(↑) patients compared to both the NGT and GDM/SIRT1(↔) groups and non-significantly up-regulated in the GDM/SIRT1(↔) subjects compared to NGT controls, indicating that the effect of ACLY expression may be distinct in leukocytes, depending on the SIRT1 expression status in these cells.