SIRT1 and gestational diabetes: Third, we confirmed a significant expression of four out of six genes identified by the PCR array approach in an extended GDM/SIRT1(↑) samples, indicating that moderate up-regulation of leukocyte SIRT1 expression (three-fold) in the hyperglycemic GDM/SIRT1(↑) group works in concert with significant up-regulation (IL6, G6PD, and SNAP23) and down-regulation (ACLY) of diabetes-related genes involved in cell metabolism, inflammation, and molecular transport and trafficking.