SIRT1 and obesity due to melanocortin 4 receptor deficiency: Interestingly, PCR analyses indicated that the leukocyte SIRT1 mRNA level was significantly higher in the hyperglycemic GDM group compared to the NGT group (1.32-fold difference between the groups; p = 0.041), even after adjusting for age and obesity parameters, such as pre- and pregnancy BMI and gestational body weight gain (Table 3).