The next stage examined whether gene expression changes for the six selected genes with an FC of <−2 or >2 (SNAP23, G6PD, IL6, IRS2, ACLY, SREBF1) in the pooled RNA samples of the GDM/SIRT1(↑) patients in a PCR profiler array could be representative for this group in general. This evidence concerns the gene SIRT1 and gestational diabetes.