SIRT1 and gestational diabetes: To achieve this, RT-qPCR was used to compare the expression levels of the six gene transcripts in the three groups: RNA samples (n = 30) from GDM/SIRT1(↑) individuals versus RNA samples (n = 90) from GDM/SIRT1(↔) patients versus RNA samples (n = 41) from NGT subjects (Table 5 and Figure 2).