Therefore, our research strategy was based on the identification of genes with differential expression (2-fold or more) by PCR array expression profiling of leukocytes from the small number of GDM/SIRT1(↑) patients, followed by verification of their expression in a relatively large number of diabetic patients of the GDM/SIRT1(↑) and GDM/SIRT1(↔) groups, thus improving the accuracy and reliability of our results. This evidence concerns the gene SIRT1 and gestational diabetes.