SIRT1 and gestational diabetes: Since quantitative and qualitative differences were seen in the transcriptional expression pattern of leukocytes between the pooled RNA samples of GDM/SIRT1(↑) and NGT women, the next stage more closely examined the PCR array data in the entire experimental groups of GDM/SIRT1(↑) (n = 30) versus NGT (n = 41).