DUOX1 and immune system toxicity: To assess the mechanism underlying the protective effect of WPE on FNT induced immunotoxicity, we evaluated the expression of NOX-2 and DUOX-1 on splenocytes treated with FNT alone or in combination with WPE for 48 h in the presence of Con A. As shown in Figure 7A,B, WPE at 1–10 μg/mL significantly reduced NOX-2 and DUOX-1 overexpression induced by FNT in Con A stimulated splenic T-cells in a concentration dependent manner.